In this study, gene expression profiling, DNA copy number detection, and exome sequencing using formalin-fixed samples from 18 patients with mixed HCC-CCA were performed, encompassing the whole histological spectrum of the disease. The main conclusions were that mixed HCC-CCA represents a heterogeneous group of tumors, with the stem-cell type characterized by features of poor prognosis, and the classical type with common lineage for HCC and iCCA components. In addition, integrative genomic analysis of HCC-CCAs revealed that cholangiolocellular carcinoma represents a distinct biliary-derived entity compared with the stem-cell and classical types.
(Moeini A et al. – Journal of Hepatology, 23 January 2017)